Diagnosis of Trichomoniasis in Male Patients on Performing Nested Polymerase Chain Reaction

Diagnosis of Trichomoniasis in Male Patients on Performing Nested Polymerase Chain Reaction Erkek Hastalarda Trichomoniasis Tanısında Polimeraz Zincir Reaksiyonu (Pzr) Kullanımının Etkinliğinin Araştırılması

Objective: Trichomoniasis is a parasitic infection that occurs with the settlement of Trichomonas vaginalis in female and male urinary and reproductive tracts. This infection is generally asymptomatic in males, and males are thought to be a carrier for the transmission of infection. In this study, our aim was to detect trichomoniasis using nested polymerase chain reaction among males who were r...

Diagnosis of bacteremia in patients with suspected septicemia using polymerase chain reaction method

Background: Sepsis or blood stream infection is a clinical lethal syndrome with severe systemic inflammatory response to infection, if not treated quickly, is associated with dangerous consequences and high morbidity and mortality. The traditional and conventional method for identification of sepsis is blood culture method which is so time-consuming and long that it eliminates the possibility o...

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Clinical usefulness of the nested polymerase chain reaction in the diagnosis of extrapulmonary tuberculosis.

OBJECTIVE To evaluate the effectiveness of nested polymerase chain reaction (PCR) for diagnosis of extrapulmonary tuberculosis (ETB), as well as the impact of PCR results on clinical management. MATERIALS AND METHODS We conducted a study of nested PCR tests in 45 patients and a review of patient hospital files, calculating sensitivity, specificity, positive predictive value (PPV), and negativ...

evaluation of multiplex nested polymerase chain reaction for routine hepatitis c virus genotyping in egyptian patients